Postdoc position – The role of non-coding RNAs in shaping 3D chromatin architecture in stem cells

    Website Istituto Italiano di Tecnologia

    Contract: Postdoc position- The role of non-coding RNAs in shaping 3D chromatin architecture in stem cells
    This open position is financed by the Human Technopole (within the Early Career Fellowship Program). The successful Post-doc candidate will work under the supervision of Dr. Dafne Campigli Di Giammartino.

    Date posted: 15 March 2022
    Deadline: 30 June 2022

    About Organization: At IIT we work enthusiastically to develop human-centered Science and Technology to tackle some of the most pressing societal challenges of our times and transfer these technologies to the production system and society. Our Genoa headquarter is strictly inter-connected with our 11 centers around Italy and two outer-stations based in the US for a truly interdisciplinary experience.
    Website: https://www.iit.it/it

    Job Role: You’d be working in a multicultural and multi-disciplinary group, where computational scientists, chemists, biotechnologists and biologists collaborate, each with their own expertise, to carry out common research within the Non-coding RNAs and RNA-based therapeutics Research Line.
    Our research focuses on understanding how  non-coding elements (DNA/RNA) and their epigenetic modifications regulate 3D genome architecture and  gene expression in stem cells and cancer stem cells. To address this question we use cutting-edge chromatin conformation assays such as Hi-C and Hi-ChIP and other high-throughput sequencing techniques (e.g. RNA-seq, ChIP-seq, ATAC-seq) in combination with CRISPR-based genetic and epigenetic engineering tools in both mouse embryonic stem cells and  human cancer stem cells. Our final goal is to understand how ncRNAs and their protein co-factors can induce and/or maintain 3D promoter-enhancer contacts, which ultimately determines the set of cell-type-specific expressed genes that are important for stemness and tumorigenesis.

    Requirements:

    • A PhD in Biology including molecular biology, genetics, bioinformatics, computer science and/or similar disciplines
    • Previous experience in stem cell culture and molecular biology techniques
    • Experience in producing and analyzing genome-wide technologies, including but not limited to CHIP-seq, RNA-seq, ATAC-seq etc
    • A strong publication record
    • The ability to properly report, organize and publish research data
    • Documented experience in coaching junior scientists

    Preferences would be given if:

    -Experience in 3D chromatin conformation assays  (e.g. Hi-C and Hi-ChIP etc).
    -Previous research experience in RNA biology (non-coding RNAs is a plus).
    -Ability to perform genomic and epigenomic editing techniques in stem cells using CRISPR-(d)Cas9 technologies.
    -Ability to analyze and visualize sequencing data and integrating multi-omics data produced by different methods using computational tools and pipelines.

    Responsibilities: 

    • Perform experiments in the area of 3D chromatin conformation, stem cells, non-coding RNAs and epitranscriptomics.
    • Use advanced molecular biology techniques for the manipulation of  RNA, DNA and proteins.
    • Analyze and integrate next-generation sequencing data using computational tools and pipelines.
    • Record experimental data in both paper and electronic format.
    • Assist in generating preliminary data and figures for grant proposals.
    • Collaborate with other researchers to implement new techniques.
    • Supervise and mentor junior members of the team.
    • Write research and review articles as well as apply for external postdoctoral funding.

    To apply for this job please visit iit.taleo.net.